Journal: International Journal of Molecular Sciences

Article Title: Metabolic Shift of an Isogenic Strain of Enterococcus faecalis 14, Deficient in Its Own Bacteriocin Synthesis, as Revealed by a Transcriptomic Analysis

doi: 10.3390/ijms21134653

Figure Lengend Snippet: Antimicrobial activity of supernatants of E. faecalis 14 wild-type, its isogenic Δ bac mutant and Δ bac-Comp complemented strains against L. innocua at 6 h of growth on GM17 medium. ∆bac: E. faecalis 14 mutant deleted in ddA and ddB bacteriocin structural genes. If present, the inhibition zone indicated the susceptibility of the bacterial lawn ( L. innocua ) to the produced EntDD14 bacteriocin. The data are representative of at least three independent experiments.

Article Snippet: Cytotoxicity of E. faecalis 14 wild-type and its Δ bac derivative mutant strains was assessed in vitro using the Cell Counting Kit-8 (CCK-8) assay (Dojindo Molecular Technology, Kumamoto, Japan), for Caco-2 cells [ ].

Techniques: Activity Assay, Mutagenesis, Inhibition, Produced

Journal: International Journal of Molecular Sciences

Article Title: Metabolic Shift of an Isogenic Strain of Enterococcus faecalis 14, Deficient in Its Own Bacteriocin Synthesis, as Revealed by a Transcriptomic Analysis

doi: 10.3390/ijms21134653

Figure Lengend Snippet: Differentially expressed genes (DEGs) in the E. faecalis 14 Δ bac mutant strain. DEGs with fold change (FC) >2.0 and <0.5 of individual Δ bac (1–3) vs. mean of the wild-type (WT) were represented. The putative functional groups are indicated on the right of the figure.

Article Snippet: Cytotoxicity of E. faecalis 14 wild-type and its Δ bac derivative mutant strains was assessed in vitro using the Cell Counting Kit-8 (CCK-8) assay (Dojindo Molecular Technology, Kumamoto, Japan), for Caco-2 cells [ ].

Techniques: Mutagenesis, Functional Assay

Journal: International Journal of Molecular Sciences

Article Title: Metabolic Shift of an Isogenic Strain of Enterococcus faecalis 14, Deficient in Its Own Bacteriocin Synthesis, as Revealed by a Transcriptomic Analysis

doi: 10.3390/ijms21134653

Figure Lengend Snippet: Growth curves of E. faecalis strains in GM17 medium. E. faecalis 14 WT, Δ bac mutant and the Δ bac complemented strain. The vertical bars represent the standard deviations. The data are the means of three independent experiments. The asterisk (*) indicates that the growth rate is significantly different from that of the WT strain using the Student t test.

Article Snippet: Cytotoxicity of E. faecalis 14 wild-type and its Δ bac derivative mutant strains was assessed in vitro using the Cell Counting Kit-8 (CCK-8) assay (Dojindo Molecular Technology, Kumamoto, Japan), for Caco-2 cells [ ].

Techniques: Mutagenesis

Journal: International Journal of Molecular Sciences

Article Title: Metabolic Shift of an Isogenic Strain of Enterococcus faecalis 14, Deficient in Its Own Bacteriocin Synthesis, as Revealed by a Transcriptomic Analysis

doi: 10.3390/ijms21134653

Figure Lengend Snippet: Transmission electron micrographs of E. faecalis 14 and its Δ bac mutant strain. No significant differences in the size or organization of bacterial cells were observed.

Article Snippet: Cytotoxicity of E. faecalis 14 wild-type and its Δ bac derivative mutant strains was assessed in vitro using the Cell Counting Kit-8 (CCK-8) assay (Dojindo Molecular Technology, Kumamoto, Japan), for Caco-2 cells [ ].

Techniques: Transmission Assay, Mutagenesis

Journal: International Journal of Molecular Sciences

Article Title: Metabolic Shift of an Isogenic Strain of Enterococcus faecalis 14, Deficient in Its Own Bacteriocin Synthesis, as Revealed by a Transcriptomic Analysis

doi: 10.3390/ijms21134653

Figure Lengend Snippet: Adhesion of E. faecalis 14 and its Δ bac mutant strain to polystyrene plates as determined by OD 630nm measurements. The absorbance values are the means of three independent experiments. Sterile GM17 was used as control. The error bars represent the standard deviations. Columns with different letters are significantly different using one-way ANOVA with Tukey test and Student t test for pairwise comparisons. (*), p < 0.05; (**), p < 0.01.

Article Snippet: Cytotoxicity of E. faecalis 14 wild-type and its Δ bac derivative mutant strains was assessed in vitro using the Cell Counting Kit-8 (CCK-8) assay (Dojindo Molecular Technology, Kumamoto, Japan), for Caco-2 cells [ ].

Techniques: Mutagenesis, Sterility, Control

Journal: International Journal of Molecular Sciences

Article Title: Metabolic Shift of an Isogenic Strain of Enterococcus faecalis 14, Deficient in Its Own Bacteriocin Synthesis, as Revealed by a Transcriptomic Analysis

doi: 10.3390/ijms21134653

Figure Lengend Snippet: Cytotoxicity of E. faecalis 14 and its Δ bac mutant on Caco-2 cells (24 h). Data are means of three independent experiments. Error bars represent standard deviations. Columns with different letters are significantly different using one-way ANOVA with Tukey test and the Student t test for pairwise comparisons. (*), p < 0.05; (**), p < 0.01; not significant (n.s.), p > 0.05.

Article Snippet: Cytotoxicity of E. faecalis 14 wild-type and its Δ bac derivative mutant strains was assessed in vitro using the Cell Counting Kit-8 (CCK-8) assay (Dojindo Molecular Technology, Kumamoto, Japan), for Caco-2 cells [ ].

Techniques: Mutagenesis

Journal: Cancers

Article Title: Targeting the MDM2-p53 Interaction with Siremadlin: A Promising Therapeutic Strategy for Treating TP53 Wild-Type Chronic Lymphocytic Leukemia

doi: 10.3390/cancers17020274

Figure Lengend Snippet: Mean IC 50 concentrations of HDM201 for the panel of B cell lines.

Article Snippet: Human B cell lines, including Nalm-6, OCI-Ly3, and HAL-01 (wild-type TP53 ), as well as Ramos, Raji, and Pfeiffer ( TP53 mutant), were acquired from authenticated cell line repositories (ATCC or DSMZ).

Techniques: Mutagenesis